The multiplex fluorescent detection and chemiluminescent imaging capabilities of the Quantitative Western Blot Imaging FluorChem Q system provide a complete solution for quantitative Western blot imaging and analysis. The open platform is compatible with a wide variety of dyes and kits, providing the flexibility needed for multiple applications.
The FluorChem Q system allows quantitative detection of multiple proteins in a single assay, eliminating the need to strip and re-probe blots. Intensity values are easily normalized to a loading control to correct for inaccuracies.
Composite image of a three-color fluorescent Western blot, carcino embryonic antigen (CEA), transferrin and IgG were detected with fluorescently labeled antibodies, and imaged in three spectrally unique fluorescent channels. Imaging time: 24 seconds.
Overlapping proteins such as phosphorylated and unphosphorylated isoforms or other proteins with similar migration can be probed in the same blot. Our unique Channel Viewer feature allows visualization of individual bands in overlapping areas.
The FluorChem Q system delivers the wide dynamic range and sensitivity essential to accurately detect subtle changes in protein levels.
Serial dilutions of transferrin detected by Western blot with a secondary Cy5 labeled antibody and imaged on the FluorChem Q system. A lower limit of 0.6 pg transferrin was detected.
AlphaView Software is intuitive and easy to navigate for quantitative analysis. A pplication driven acquisition, experiment-based analysis protocols, and comprehensive annotation tools allow rapid generation of publicationready results and images.
