|
Multiplexdetectionmethodologies are rapidlybecomingpopularfor Westernblotimagingduetotheirabilitytoproviderapidquantitation acrossmultipleproteinsamples. Inthis article,wediscussthepower FluorChem Q, a CCD – based detection platform able to perform traditional chemiluminescent detection and multicolor fluorescent detection, makes fluorescentWestern imaging rapid and easy. Data analysis of multicolorWesternsisstreamlinedwith AlphaView™ Q software, a software package that providesthe ability to normalize to lane loading controls and to easily calculate fold change between samples.Together,theFluorChemQandAlphaViewQsimplify the process from image acquisition to image analysis, providing a powerful tool for imaging and analyzing multicolor Westerns.
|